To determine whether the loss of tumor-suppressive activity of BAF180 in H2 ccRCC cell lines is due to the loss of HIF1A gene expression, the H2 ccRCC cell line, 786-O, which normally expresses full-length BAF180, but lacks HIF1α expression, was stably transfected with a mouse HIF1A complementary DNA (cDNA) expression vector (BAF180 WT/HIF1A cDNA; Figure 4a). This evidence concerns the gene PBRM1 and neoplasm.