HIF1α re-expression reduced 786-O cell proliferation/survival compared with parental 786-O cells (Figures 4b and c), consistent with the reported tumor-suppressive role of HIF1α in ccRCC cell lines.24, 25 Next, BAF180 was knocked out via CRISPR/Cas9 system to generate a cell line, BAF180 KO/HIF1A cDNA (Figure 4a). The gene discussed is PBRM1; the disease is nonpapillary renal cell carcinoma.