PBRM1 and nonpapillary renal cell carcinoma: Expression of a BAF180 single-guide RNA #1 (sgRNA#1) and Cas9 nuclease are expected to introduce double-stranded DNA breaks and subsequently mutation in exon 1 of the BAF180 gene, a location where BAF180 mutations are frequently observed in primary ccRCC tumors7, 10, 11 (Figure 3g).