Another suggested mode of MDSC-mediated suppression is through interactions occurring between PD-1 on T cells and PD-L1 on MDSC.42 Given the recent interest in blockade of the PD-1/PD-L1 axis as a cancer therapy approach,43 we investigated PD-L1 expression on culture-generated MDSC, and tested whether PD-L1 had a functional role in suppressing T cells in the co-culture assay. This evidence concerns the gene CD274 and cancer.