To further evaluate tumor-targeting efficiency, T cells transfected with anti-EpCAM RNA CAR or mGFP RNA CAR were co-cultured with above tumor cell lines and IFNγ secretion was determined by ELISPOT assay since Th-1 cytokine IFNγ secretion is associated with antitumor activity of T cells for adoptive immunotherapy. This evidence concerns the gene NELFCD and neoplasm.