Co-culture of BV-EpCAM transduced tumor cells with anti-EpCAM RNA CAR T cells stimulated more significant IFNγ secretion as compared to the co-cultures of the tumor cells transduced with BV-Pax6 transduced tumors and wildtype tumor cells (Supplementary Figure 5B), indicating an EpCAM expression level-related effect of anti-EpCAM RNA CAR. This evidence concerns the gene PAX6 and neoplasm.