We found that miR-30c-5p was able to suppress FASN, the crucial enzyme in fatty acid biosynthesis and delivery of exogenous miR-30c-5p by rAAV was sufficient to attenuate triglyceride accumulation and liver steatosis in the db/db mice, suggesting a new therapeutic strategy against NAFLD. This evidence concerns the gene FASN and metabolic dysfunction-associated steatotic liver disease.