ASB3 and neoplasm: Using next-generation sequencing (for 50 cases) and Sanger sequencing assays (for 83 new cases and 3 cases with ASB3 mutations found in next-generation sequencing assays), we analyzed the ASB3 gene mutations in tumor tissues collected from 133 CRC patients treated at Sun Yat-sen University Cancer Center, and found that ASB3 gene had a high frequency of somatic mutations (5.3%, 7/133).