ANXA5 and neoplasm: Last, to test substrate concentration limitations of CalceinAM at a 5 μM concentration over the 10-day experiment, we flushed the system from the re-circulating Calcein AM and CF594-AnnexinV dyes and reintroduced newly made 5 μM CalceinAM Green and 5 μM CF594-AnnexinV to the existing, previously exposed, FNAB tumor fragments in channels and also added fresh FNAB fragments to the channel.