In this study, we show that prostate cancer-specific hypermethylation of the eight genes AOX1, CCDC181 (C1orf114), GABRE, GAS6, HAPLN3, KLF8, MOB3B, and SLC18A2 can be detected by qMSP with very high sensitivity and specificity in scarce prostate needle biopsy samples taken at the time of diagnosis. The gene discussed is CCDC181; the disease is prostate cancer.