In this study, we show that PC-specific hypermethylation of AOX1, CCDC181, GABRE, GAS6, HAPLN3, KLF8, MOB3B, SLC18A2, and GSTP1 can be detected by qMSP even in scarce prostate tissue samples from diagnostic needle biopsies. This evidence concerns the gene HAPLN3 and pachyonychia congenita.