In this study, we show that PC-specific hypermethylation of AOX1, CCDC181, GABRE, GAS6, HAPLN3, KLF8, MOB3B, SLC18A2, and GSTP1 can be detected by qMSP even in scarce prostate tissue samples from diagnostic needle biopsies. Here, GAS6 is linked to pachyonychia congenita.