KLK3 and pachyonychia congenita: Moreover, the significant positive association between methylation levels in PC biopsies and D’Amico risk score for all candidate genes, is also in agreement with previous findings from RP specimens, where high methylation levels were generally associated with at least one adverse clinicopathological factor (high PSA, high Gleason score, positive surgical margins, and/or advanced pT-stage)20, 21, 23, 43.