The results indicated that both the two groups showed higher betatrophin levels in women with GDM (≥ 30 years: SMD = 0.96; 95% CI: 0.12–1.8, P = 0.020; < 30 years: SMD = 1.29; 95% CI: 0.84–1.74, P < 0.010). Here, ANGPTL8 is linked to gestational diabetes.