We identified virulence factor genes associated with biofilm formation including aggregation substance (agg), enterococcal surface protein (esp), adhesion of collagen from E. faecalis (ace), gelatinase (gelE), endocarditis and biofilm-associated pili genes (Ebp) and sortase A (SrtA) [35, 45–48]. The gene discussed is ACE; the disease is endocarditis.