In this study, we found for the first time that members of the miR-26 family (miR-26a and miR-26b, miR-26a/b) can act as potential autophagy inhibitors to sensitize HCC cells to doxorubicin (Dox) and promotes apoptosis by directly inhibiting the expression of serine/threonine protein kinase ULK1, a critical initiator of autophagy. Here, ULK1 is linked to hepatocellular carcinoma.