Figure 5D shows that the panel provided a higher diagnostic accuracy of HCC, and the AUC was up to 0.975, with a sensitivity of 95.00%, specificity of 95.00% and accuracy of 95.00% (Table 2), which improved 7.5%, 1.25% and 4.37% compared with AFP alone, respectively. This evidence concerns the gene AFP and hepatocellular carcinoma.