To dissect the stromal and neoplastic compartment, we further generated primary CAF lines (n=2) from murine pancreatic tumours as well as PSCs (n=3) from healthy B6 mice showing typical spindle-shaped morphology, and strong expression of α-SMA, SPARC and fibronectin (figure 4A and online supplementary figure S1B). This evidence concerns the gene ACTA1 and pancreatic neoplasm.