In addition, the inhibition of proliferation and migration was further detected in TE1 and TE13 cells after treatment with 5-Aza-Dc or stable transfection of DACT1 or DACT2 plasmid, indicating the tumor suppressor role of DACT1 and DACT2 in esophageal cancer cell lines. This evidence concerns the gene DACT2 and esophageal cancer.