We then use the siRNA to konck down the Smad2/3 expression (Supplementary Figure 1C), cck8 showed that the growth of HCC cells was inhibitied more significantly (Supplementary Figure 1D), it indicated that TGF-β1/smad2/3 promoted the HCC cells growth, while the Hippo pathway inhibited the HCC cells growth and the inhibiton effect was stronger than the promotion effect of TGF-beita/smad2/3. The gene discussed is SMAD2; the disease is hepatocellular carcinoma.