To examine whether this was because the expression of ACP2 induced chemosensitivity, we used the shACP2 lentivirus to knock down ACP2 expression in human CRC cell lines HCT-116 and DLD1 after we examined the expression level of ACP2 in six colon cancer cell lines (Supplementary Figure 1). This evidence concerns the gene ACP2 and malignant colon neoplasm.