In this study, we demonstrate that DP2 can upregulate uPA and uPAR expression via TLR2/4-mediated ERK activation, reinforce uPAR binding to integrin, and subsequently activate integrin/FAK signal transduction, resulting in the promoted cell motility and invasive ability of NSCLC cell A549. This evidence concerns the gene TLR2 and non-small cell lung carcinoma.