Except for the expression of MYPT1 in all other cases, the protein expression as well as the phosphorylation of PRMT5 at Thr80, and the arginine methylation of histone 2A and 4 increased in the higher grade and stage in hepatocellular carcinoma tissues (n = 11–13) exhibiting a strong correlation to the grade and state of the tumor. Here, PRMT5 is linked to hepatocellular carcinoma.