Based on the phosphorylation of MYPT1T850 we confirmed that the activity of the nuclear MP decreased, the activity of PRMT5 phophorylated at Thr80 increased and PRMT5-specific repressive R3-dimethylation increased in HCC and in other cancer cell lines suggesting the fundamental role of this pathway in tumorigenesis. Here, PRMT5 is linked to hepatocellular carcinoma.