Tumor tissue lysates obtained from a large group of human patients with grade 2 and 3 and state II and III (Fig. 5 dark grey columns) hepatocellular carcinoma (n = 20), four different types of metastatic liver cancers (Fig. 5 light grey columns) as well as 15 other types of cancer cell lines with their adequate normal cell type controls (Fig. 5 black columns) were analysed using high throughput screening for multiple protein targets such as PRMT5 (Fig. 5A), pPRMT5T80 (Fig. 5B), MYPT1 (Fig. 5C), pMYPT1T850 (Fig. 5D), histone 2A (Fig. 5E) and histone 2A symmetric arginine methylation (Fig. 5F). Here, PRMT5 is linked to hepatocellular carcinoma.