EIF2AK2 and Zika virus infectious disease: Polysome profiles and immunofluorescence analyses of Huh7 PKR ko cells (Fig. S6E and F), as well as Huh7 GADD34-expressing cells (Fig. S7F and G), confirmed that both SG-like focus formation and translation repression upon ZIKV infection do not require the PKR-eIF2α signaling pathway.