To further confirm the results of the screen and to determine whether regulation of JMJD2B by p53 also occurred in different cancer cell lines, we analyzed the expression of JMJD2B by qPCR in cells transiently transfected with siRNA duplexes targeting p53 (si-p53) or a non-targeting control siRNA (siCON), and treated with doxorubicin. Here, TP53 is linked to cancer.