In RAW 264.7 macrophages infected with M. smegmatis expressing wild-type PPE2 (Msmeg-PPE2), PPE2 was found to be colocalized with an early endosome marker EEA1 at around 1 hour post-infection, but at 6 hour post-infection, most of the PPE2 protein was found to be localized in cytoplasm as evident from its co-localization with a cytoplasmic marker tubulin (Fig. S3A). The gene discussed is EEA1; the disease is infection.