To further test that p53-independent actions of BITC are not limited to MDA-MB-231 cells, we treated multiple p53-mutant breast cancer cells with BITC and found that BITC could inhibit growth and clonogenicity of MDA-MB-468, BT474, T47D and Hs578t cells (Fig. 3G,H). Here, TP53 is linked to breast cancer.