To determine whether PKM2 particularly p-PKM2 plays a role in modulating the release of exosomes from tumour cells, we assessed exosome release after knocking down PKM2 level in A549 and Hela tumour cells via PKM2 siRNA or overexpressing PKM2 in myoblasts and MEC via HA-tagged PKM2-expressing plasmid. This evidence concerns the gene PKM and neoplasm.