To test for an association of MCH and OX gene expression with the protective effect of sleep deprivation, time course of gene expression of Pmch and OxA and their receptors Mchr1, Ox1R and Ox2R were analysed at 4, 12, 24 hours and at 3, 5, 7 days following ischemia in both ischemic and contralateral hemispheres (Fig 7A). This evidence concerns the gene MCHR1 and ischemia.