Compared with the control, overexpression of RCAN1 renders more glioma cells death, a 3.33± 0.41% apoptosis ratio relative to 1.73 ± 0.27% of control by TUNEL staining in U251 cells, and a 3.98± 0.17% apoptosis ratio relative to 1.91 ± 0.24% of control by TUNEL staining in T98G cells (p < 0.05, Figure 4A and 4B, lane 2 versus lane 1). The gene discussed is RCAN1; the disease is glioma.