Therefore, in contrast to the study of Baron Toaldo et al., who quantified ROIs enclosing the whole tumor, in the present study ROIs were selected over the vital outer rim of therapy and control tumors to avoid tumor necrosis and areas of elevated interstitial pressure with consecutively altered contrast media kinetics with the aim to exclude tumor necrosis as potential confounding factor for the observed reduction of VEGFR-2 expression. This evidence concerns the gene KDR and neoplasm.