To clarify the effect of disease severity on blood TDPase, TMPase, and TPK activities, AD patients were divided into different subgroups according to their MMSE (severe subgroup: MMSE<10, mild to moderate subgroup: MMSE ≥ 10) or ADL scores (severe subgroup: ADL<23, mild to moderate subgroup: ADL ≥ 23). The gene discussed is ACP3; the disease is Alzheimer disease.