Although TSA treatment alone did not significantly affect PI3K/AKT/mTOR pathway protein phosphorylation, if in combination with BEZ235, it further inhibited the phosphorylation of S6(S240/244), Akt (S473) and 4EBP1 (S65) in various degree in breast cancer cells relative to single treatments. This evidence concerns the gene AKT1 and breast cancer.