To investigate the function change of MUC4/Y gene without or with domain-lacking in pancreatic cancer, PANC-1 cells, which do not express endogenous MUC4 [32], were stable transfected by target genes, and selected using 10% DMEM containing puromycin (2.0 μg/mL), respectively. The gene discussed is MUC4; the disease is familial pancreatic carcinoma.