RB1 and neoplasm: In the genomes of 15 EGFR-amplified tumours, we queried the presence of alterations at CCND1 and related cell cycle regulators, identifying co-occurring oncogenic alterations localizing of CCND1, CDKN2A, CDK6 and RB1 (Fig. 4a) in addition to EGFR. Among these, the most notable secondary alteration was the focal amplification at chromosome 11q13, at the locus of CCND1, in 10 samples (66.7%).