SOD1 and amyotrophic lateral sclerosis: To further check the specificity of our anti-SOD1int antibody in vitro, we prepared various forms of WT and ALS-mutant (A4V, G37R, and G85R) SOD1 proteins including E,E-SOD1SH, E,E-SOD1S-S, E,Zn-SOD1SH, E,Zn-SOD1S-S, Cu,E-SOD1S-S, Cu,Zn-SOD1S-S, and soluble oligomers with disulfide crosslinks (see Fig. 1a).