Among 109 up-regulated genes, 11genes (BMP2, CCL26, CCL8, EPAS1, EPB41L3, FGF7, GAS1, LEF1, PF4V1, PRKG2 andPTGS2) were mapped to 7 significant pathways (corrected gamma p-value < 0.05), including Adherens junction, Melanoma, Cytokine-cytokine receptor interaction, Pathways in cancer, Hedgehog signaling pathway, Tight junction, and Long-term depression (Additional file 1: Table S5 and Fig. 1d). The gene discussed is EPAS1; the disease is melanoma.