In subsequent studies, it was shown that the selection criteria should not be as restrictive as the Milan criteria, and a number of extended criteria have been proposed, such as the University of California San Francisco (UCSF) criteria by Yao et al. in 2001 [6], the Hangzhou criteria (total tumor diameter ≤ 8 cm; or total tumor diameter > 8 cm, with histopathologic grade I or II and pre-operative AFP level ≤ 400 ng/mL, simultaneously) by Zheng et al. in 2008 [7], and the up-to-seven criteria by Mazzaferro et al. in 2009 [8], respectively. This evidence concerns the gene AFP and neoplasm.