Next, to allow direct analysis of the role of PKD1 inhibition in human metastatic melanoma, M2 cell clones stably expressing shRNAs directed against PKD1 (named P2, P3, P4, P5, P6 and P7) or scrambled shRNA control (named C1 and C2) were generated by lentiviral particles transduction. The gene discussed is PKD1; the disease is metastatic melanoma.