Supplementary Figure S4 shows the incidence of tumoral lesions per site for each mouse considered in our experiment. Macroscopic dissection and bioluminescence measurement analysis of metastatic sites confirmed luciferase detection corresponding to the presence of tumor cells (Figure 3C). Goldner coloration of the collected bone samples confirmed bone tumor localisation. A histological analysis with antibodies against ERG and Ki67 respectively confirmed the TMPRSS2-ERG status and the high rate of proliferation (Figure 3E and Supplementary Figure S5). Here, ERG is linked to bone neoplasm.