In addition, the apoptotic cells were increased about three-fold in each colon cancer cell line by knockdown of REP1 after 72 h under starvation (Figure 2d) and, consistently, the levels of pro-apoptotic molecules such as BIM and BAK, and CDK inhibitor p27, were also increased (Figure 2e). This evidence concerns the gene BAK1 and malignant colon neoplasm.