This apoptosis pathway involves the participation of Bcl-2 family proteins, including the proapoptotic protein Bax and the antiapoptotic protein Bcl-2.33 In this study, Hoechst 33358 staining and FCM assays both revealed that nifuroxazide treatment induced apoptosis on CRC cells in a concentration-dependent manner. This evidence concerns the gene BAX and colorectal carcinoma.