IHC staining results show that phosphorylation levels of both HK1-Y732 and c-Src in colon adenocarcinoma (Fig. 8e) and breast duct carcinoma (Fig. 8f) are obviously higher than that in corresponding cancer-adjacent normal tissues, meanwhile the intensities of p-c-Src and HK1-p-Y732 are highly correlated with a correlation coefficient of 0.9317 for colon adenocarcinoma and 0.9027 for breast duct carcinoma. The gene discussed is HK1; the disease is colon adenocarcinoma.