CASP3 and congestive heart failure: As for the relationship between apoptosis and CHF, we used immunohistochemistry assays and western blot methods which belonged to quantitative and qualitative analysis, to detect the levels of apoptotic and antiapoptotic proteins, such as Bax, Bcl-2, and caspase-3, they are on the downstream of apoptotic signal pathway, and they mediate the final morphological and biochemical alterations which are characteristic of apoptosis [24].