To determine the effects of panobinostat on the growth, survival, and death of brainstem glioma (BSG) cells, we generated H3.3-K27M-expressing tumors by injecting neonatal Nestin-tv-a (Ntv-a);p53-fl/fl mice with RCAS-PDGF-B, RCAS-H3.3-K27M, and RCAS-Cre viruses as described in [22] and in Materials and Methods. The gene discussed is NES; the disease is brain stem glioma.