In this model, mice exhibit symptoms of brain tumor formation within 60–80 days post tumor cell implantation and harbor tumors that histologically resemble human DIPG, with tumor cells infiltrating the brainstem as seen with routine hematoxylin and eosin staining and the cerebellum as shown by vimentin staining (Fig 6B). Here, VIM is linked to diffuse intrinsic pontine glioma.