To confirm this data, we immunohistochemically (IHC) stained adjacent sections from a breast cancer cohort (n=326, of all subtypes) with a mouse monoclonal anti-IRIS antibody raised against the intron 11 domain of IRIS (does not cross react with BRCA1 [35]) and a mouse monoclonal anti-BRCA1 antibody raised against the very C-terminal sequence of exon 24 of BRCA1 (does not cross react with IRIS [35]) on adjacent sections. The gene discussed is BRCA1; the disease is breast cancer.