To investigate whether the HMA-resistance-specific CNAs influence gene expression, we assayed the expression of 8 cancer-related genes by quantitative reverse transcription PCR (RT-qPCR) including BCL9, ARNT, ABL2, STK11, TCF3, SMARCA4, RUNX1, and U2AF1 (Figure 4). This evidence concerns the gene U2AF1 and cancer.