Consistent with the previous reports in CRC samples33, 34, we also found that transcription of downstream target genes of Wnt signalling, including CCND1 and c-MYC, increased markedly in tumour samples, whereas Axin2 was somewhat, but not significantly, upregulated in the CRC samples (Fig. 2b–d). Here, AXIN2 is linked to colorectal carcinoma.