In the present study, the overall results showed that SPO11 gene polymorphism could increase the risk of male infertility (TT + CT vs. CC: OR = 4.14, 95%CI = 2.48–6.89; CT vs. CC: OR = 4.34, 95%CI = 2.56–7.34; T vs. C: OR = 4.35, 95%CI = 2.58–7.34). This evidence concerns the gene SPO11 and male infertility.