With the intent to address the prevalence of HOXB13 mutations among prostate cancer patients of the Portuguese population, we have recently sequenced the entire HOXB13 coding region in 462 patients with early-onset and/or familial/hereditary prostate cancer and found two novel missense mutations – c.383C>A, p.(Ala128Asp) (A128D) and c.720C>A, p.(Phe240Leu) (F240L) – predicted to affect protein function by in silico analysis [17]. Here, HOXB13 is linked to prostate carcinoma.