A 3.6 cM (1.8 Mb) interval is shared between four PPCD families that demonstrated linkage to the pericentromeric region of chromosome 20.[13, 15–20] However, mutational analysis of the coding sequence and exon-intron boundaries of the 32 positional candidate genes from the shared interval failed to identify a pathogenic mutation in one of the four families linked to the PPCD1 locus.[15–17, 19–23] Given this, we employed a targeted next-generation sequencing approach to identify potentially pathogenic non-coding variants in these 32 positional gene candidates. The gene discussed is OVOL2; the disease is posterior polymorphous corneal dystrophy.