DLX2 encodes a homeodomain-containing transcription factor and has been implicated in the generation of GABAergic cortical interneurons.47 A previous study examining DNA methylation changes in post-mortem PFC brain samples from MDD patients and controls identified DNA hypermethylation at the PRIMA1 gene, and a concurrent decrease in gene expression.24 Interesting, cg23889730, a CpG site proximal to the PRIMA1 gene region assessed in the MDD post-mortem brain study, was also hypermethylated in MDD suicide cases compared with controls (Δβ=0.02, P=0.04) in BA11 but not in BA25 in our study. This evidence concerns the gene PRIMA1 and major depressive disorder.