The weak-ECM-interacting group includes active LKB1 (H1299 pLKO.1), rescued LKB1 (H157 LKB1WT), LKB1 non-kinase domain mutant (LKB1C430S), and the control silencing case (H157 LKB1WT + siRNActrl), which do not have a significant change of the local alignment over time, nor high alignment vectors from the tumor spheroid boundary outward. This evidence concerns the gene STK11 and neoplasm.