Next, because one of the critical factors recruiting macrophages in the course of inflammatory responses is monocyte chemoattractant protein 1 (MCP-1), its expression immediately (3 days) after TAC was analyzed by quantitative PCR using isolated cardiomyocytes to specifically certify the importance of myocardial afadin deletion for the recruitment of macrophages in the heart. The gene discussed is CCL2; the disease is persistent truncus arteriosus.