INS and metabolic dysfunction-associated steatohepatitis: In this study, we used a high-fat atherogenic diet (60% fat, 1.25% cholesterol, and 0.5% cholate) to induce a more severe form of NASH, as this diet induces hepatic insulin resistance, progressive steatosis, inflammation, and fibrosis over 6 to 24 weeks, mimicking the human disease pathology [47].