ALDH2 O-GlcNAc modification was increased by 1.16 fold in the HG+PUGNAc group compared with basal conditions, whereas hyperglycemia per se increased ALDH2 O-GlcNAc modification by 66.2%, indicating that PUGNAc could further increase ALDH2 O-GlcNAc modification under high glucose conditions (Figure 4A). This evidence concerns the gene ALDH2 and Hyperglycemia.